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髙橋 剛

准教授

先端工学研究チーム

生物有機化学分野

1.Uozumi R, Takahashi T, Yamazaki T, Granholm V, Mihara H.: Design and conformational analysis of natively folded β-hairpin peptides stabilized by nucleobase interactions. Biopolymers 94: 830-842, 2010.


2.Takahashi T, Ohta K, Mihara H.: Rational design of amyloid β-peptide (Aβ)-binding proteins: Pseudo-Aβ β-sheet surface presented in green fluorescent protein binds tightly and preferentially to structured Aβ. Proteins 78: 336-347, 2010.


3.Suzuki M, Takahashi T, Sato J, Mie M, Kobatake E, Mihara H.: Designed short peptides that form amyloid-like fibrils in coassembly with amyloid β-peptide (Aβ) decrease the toxicity of Aβ to neuronal PC12 cells. ChemBioChem 16: 1525-1530, 2010.


4.Miyachi A, Takahashi T, Matsumura S, Mihara H.: Peptide nanofibers modified with a protein by using designed anchor molecules bearing hydrophobic and functional moieties. Chem. Eur. J. 16: 6644-6650, 2010.


5.Shimizu T, Kanamori Y, Furuki T, Kikawada T, Okuda T, Takahashi T, Mihara H.: Sakurai M.: Desiccation-induced structuralization and glass formation of group 3 late embryogenesis abund protein model peptides. Biochemistry 49: 1093-1104, 2010.


6.Takahashi T, Tada K, Mihara H.: RNA aptamers selected against amyloid β-peptide (Aβ) inhibit the aggregation of Aβ. Mol. Biosyst. 5: 986-991, 2009.


7.Jumawid MT, Takahashi T, Yamazaki T, Ashigai H, Mihara H.: Selection and structural analysis of de novo proteins from an α3β3 genetic library. Protein Sci. 18: 384-398, 2009.


8.Sawada T, Takahashi T, Mihara H. Affinity-based screening of peptides recognizing assembly states of self-assembling peptide nanomaterials. J. Am. Chem. Soc. 131: 14434-14441, 2009.


9.Takahashi T, Ohta K, Mihara H.: Embedding the amyloid β-peptide (Aβ) sequence in green fluorescent protein (GFP) inhibits Aβ oligomerization. ChemBioChem 8: 985-988, 2007.


10.Sato J, Takahashi T, Oshima H, Matsumura S, Mihara H.: Design of peptides that form amyloid-like fibrils capturing amyloid β1-42 peptides. Chem. Eur. J. 13: 7745-7752, 2007.


11.Watanabe S, Tomizaki KY, Takahashi T, Usui K, Kajikawa K, Mihara H.: Interactions between peptides containing nucleobase amino acids and T7 phages displaying S. cerevisiae proteins. Biopolymers 88: 131-140, 2007.


12.Takahashi T, Dewi Y, Mihara H.: Utilization of L-α-nucleobase amino acids (NBAs) as a protein engineering tool: construction of NBA-modified HIV-1 protease analogues and enhancement of dimerization induced by nucleobase interaction. ChemBioChem 7: 729-732, 2006.


13.Gyurcsik B, Haruki H, Takahashi T, Mihara H, Nagata K.: Binding modes of the precursor of Adenovirus major core protein VII to DNA and template activating factor I: implication for the mechanism of remodeling of the Adenovirus chromatin. Biochemistry 45: 303-313, 2006.


14.Miyanishi H, Takahashi T, Mihara H.: De novo design of peptides with L-α-nucleobase amino acids and their binding properties to the P22 boxB RNA and its mutants. Bioconjugate Chem. 15: 694-698, 2004.


15.Yana D, Takahashi T, Mihara H, Ueno A.: A peptide-cyclodextrin hybrid system capable of detecting guest molecules utilizing fluorescence resonance energy transfer. Macromol. Rapid Commun. 25: 577-581, 2004.


16.Yamashita T, Takahashi Y, Takahashi T, Mihara H.: Inhibition of peptide amyloid formation by cationic peptides with homologous sequences. Bioorg. Med. Chem. Lett. 13: 4051-4054, 2003.


17.Matsumura S, Takahashi T, Ueno A, Mihara H.: Complementary nucleobase interaction enhances peptide-peptide recognition and self-replicating catalysis. Chem. Eur. J. 9: 4829-4837, 2003.


18.Takahashi T, Ueno A, Mihara H.: Nucleobase amino acids incorporated into the HIV-1 nucleocapsid protein increased the binding affinity and specificity for a hairpin RNA. ChemBioChem 3: 543-549, 2002.


19.Takahashi T, Hamasaki K, Ueno A, Mihara H.: Construction of peptides with nucleobase amino acids: design and synthesis of the nucleobase-conjugated peptides derived from HIV-1 Rev and their binding properties to HIV-1 RRE RNA. Bioorg. Med. Chem. 9: 991-1000, 2001.


20.Balasundaram G, Takahashi T, Ueno A, Mihara H.: Construction of peptide conjugates with peptide nucleic acids containing an anthracene probe and their interactions with DNA. Bioorg. Med. Chem. 9: 1115-1121, 2001.


21.Kumagai I, Takahashi T, Hamasaki K, Ueno A, Mihara H.: HIV-1 Rev peptides conjugated with peptide nucleic acids and their efficient binding to RRE RNA. Bioorg. Med. Chem Lett. 11: 1169-1172, 2001.


22.Takahashi T, Hamasaki K, Kumagai I, Ueno A, Mihara H.: Design of a nucleobase-conjugated peptide that recognizes HIV-1 RRE IIB RNA with high affinity and specificity. Chem. Commun. 349-350, 2000.


  1. 23.Kuribayashi H, Takahashi T, Nagata K, Ueno A, Mihara H.: Construction of two-stranded α-helix peptides based on influenza virus M1 protein selectively bound to RNA. Bioorg. Med. Chem. Lett. 10: 2227-2230, 2000.


24.Kumagai I, Takahashi T, Hamasaki K, Ueno A, Mihara H.: Construction of HIV Rev peptides containing peptide nucleic acid that bind HIV RRE IIB RNA. Bioorg. Med. Chem. Lett. 10: 377-379, 2000.



総説


  1. 1.Takahashi T, Mihara H.: Peptide and protein mimetics inhibiting the amyloid β-peptide aggregation. Acc. Chem. Res. 41: 1309-1318, 2008. (review)


  1. 2.Mihara H, Matsumura S, Takahashi T.: Construction and control of self-assembly of amyloid and fibrous peptides. Bull. Chem. Soc. Jpn. 78: 572-590, 2005. (review)


3.高橋 剛, 松村 幸子, 三原 久和.: アミロイドβペプチド(Aβ)を標的とした人工タンパク質・ペプチドの設計とAβ集合体形成の制御. 生物物理 47:228-234, 2007.


4.高橋 剛, 三原 久和.: 生体反応を司るらせん・たんぱく質のらせん構造. 化学と教育 55:356-359, 2007.


  1. 5.高橋 剛, 三原 久和.: 新しいタンパク質をデノボ創成する. 化学 59:64-65, 2004.



プロシーディング等


1.Uozumi R, Takahashi T, Mihara H.: Design of β-hairpin peptides having nucleobase amino acids (NBAs) and stabilization of structures by NBA interaction. Peptide Science 2007 (Aimoto S, Ono S. Eds.) The Japanesae Peptide Society, Osaka, pp 471-472, 2008.


2.Takahashi T, Ohta K, Mihara H.: Construction of green fluorescent protein (GFP) variants with amyloid β-peptide sequence. Peptide Science 2006 (Ishida H, Mihara H. Eds.) The Japanese Peptide Society, Osaka, pp 9 2006.


3.Takahashi T, Tada K, Mihara H.: In vitro selection of RNA aptamers that bind to the oligomeric state of amyloid β-peptide. Peptide Science 2005 (Wakamiya T. Ed.) The Japanese Peptide Society, Osaka, pp 483-484, 2006.


4.Takahashi T, Tada K, Oshima H, Matsumura S, Mihara H.: Construction of the artificial peptides and RNA targeting amyloid peptides. Peptide Science 2003 (Ueki M. Ed.) The Japanese Peptide Society, Osaka, pp 455-456, 2004.


5.Miyanishi H, Takahashi T, Ueno A, Mihara H.: De novo design of α-helical peptide with nucleobase amino acids that specifically recognize a hairpin RNA. Peptide Science 2002 (Yamada T. Ed.) The Japanese Peptide Society, Osaka, pp 369-370, 2003.


6.Tada K, Takahashi T, Mihara H.: In vitro selection of RNA aptamers that inhibit the amyloid-fibril formation of Aβ. Biomolecular Chemistry (Scientific Program Committee of ISBC 2003 Ed.) Maruzen, Tokyo, pp 20-21, 2003.


7.Takahashi T, Ueno A, Mihara H.: Construction of RNA-binding proteins having nucleobase amino acids based on HIV-1 nucleocapsid protein. Peptides: the Wave of the Future (M. Lebl and R. A. Houghten, Eds.) Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 518-519, 2002.


8.Takahashi T, Kumagai I, Hamasaki K, Ueno A, Mihara H.: Design of peptides with L-α-nucleobase amino acids and their selective recognition of RNA. Peptide Science 2000 (Shioiri T. Ed.), The Japanese Peptide Society, Osaka, pp 81-82, 2001.


9.Takahashi T, Kumagai I, Hamasaki K, Ueno A, Mihara H.: Design and synthesis of peptides containing nucleobase amino acids and their interaction with RNA. Peptide Science 1999 (N. Fujii, Ed.), The Japanese Peptide Society, Osaka, pp 397-398, 2000.


10.Balasundaram G, Takahashi T, Ueno A, Mihara H.: Synthesis of PNA-peptide conjugates and their interactions with DNA. Peptides for the New Millennium (Fields GB, Tam JP. Eds.) Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 790-791, 2000.


11.Takahashi T, Kumagai I, Hamasaki K, Ueno A, Mihara H.: Design of artificial peptides that recognize the HIV RRE IIB RNA. Nucleic Acids Symp. Ser. Oxford University Press, Oxford, 42, pp 271-272, 1999.


12.Takahashi T, Ueno A, Mihara H.: Combination of α-helix peptides with intercalator anthracene for reporting peptide-DNA interaction. Peptides; Frontiers of Peptide Science (Tam JP, Kaumaya PTP. Eds.) Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 130-131, 1999.


13.Kuribayashi H, Takahashi T, Watanabe K, Nagata K, Ueno A, Mihara H.: Interaction of peptides from influenza virus matrix protein with nucleic acids. Nucleic Acids Symp. Ser. Oxford University Press, Oxford, 39, pp 63-64, 1998.

業 績

原著論文